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Cynthia Brame

Cynthia Brame came to Vanderbilt in July of 2012, leaving her position as Associate Professor and Chair of Biology at Centenary College to join the Center for Teaching. The transition reflects Cynthia’s growing emphasis on scholarly teaching and faculty development, which grew out of her interest in course and curriculum design at Centenary.

Cynthia’s earlier research focused on lipid peroxidation products that covalently modify proteins as well as on development of mass spectrometry methods for characterization of modified proteins. When she joined the faculty at Centenary, Cynthia modified this work to facilitate involvement of undergraduates, developing a project on structural regulation of CK1 protein kinases in collaboration with Dr. Lucy Robinson. Importantly, Cynthia fused these disciplinary efforts with pedagogical research, developing course modules that allowed students to pursue authentic scientific research as part of regular courses. These interests informed her later efforts to develop interdisciplinary modules for science classes in collaboration with faculty members from computer science, philosophy, and physics, and was essential for her leadership of her department’s curriculum redesign guided by AAAS’s Vision and Change in Undergraduate Biology Education.

At the Center for Teaching, Cynthia serves as the liaison for the science and engineering departments as well as the School of Nursing. She teaches courses in Vanderbilt’s Biological Sciences department.

EDUCATION

Vanderbilt University, Nashville, TN, 1994-1999

Ph.D. in Pharmacology

Dissertation: Discovery of isolevuglandins, novel ketoaldehyde lipid peroxidation products, and characterization of their protein adducts.

Centre College, Danville, KY, 1990-1994

B.S. magna cum laude in Biochemistry and Molecular Biology

Minor in Chemistry

Finch V, Ehret C, and Brame CJ. The flipped classroom in historical and theoretic context: Where might we go from here? Submitted: Journal on Excellence in College Teaching.

Brame CJ and Biel R. Test-enhanced learning: The potential for testing to promote greater learning in undergraduate science courses. CBE-Life Sciences Education 14, 1-12, 2015.

Ortega RA and Brame CJ. The synthesis map is a multi-dimensional educational tool that provides insight into students’ mental models and promotes students’ synthetic knowledge generation. CBE- Life Sciences Education 14, 1-11, 2015.

Chick N and Brame CJ. An investigation of the products and impact of graduate student SoTL programs: Observations and recommendations from a single institution. International Journal for the Scholarship of Teaching and Learning 9, No. 1, Article 3, 2015. Available at http://digitalcommons.georgiasouthern.edu/ij-sotl/vol9/iss1/3.

Rao AS, Fan J, Brame CJ, and Landman BA. Improving conceptual understanding of signals and systems in undergraduate engineering students using collaborative in-class laboratory exercises. 2014 ASEE Annual Conference, paper ID #9790. Available at http://www.asee.org/public/conferences/32/papers/9790/view.

Brame CJ, Chiang H, Robinson LC, and Messina TM. A molecular dynamics laboratory module designed   to support a molecular genetics research project. In preparation.

Butcher GQ, Ciocchetti C, Carlisle W, Goadrich M, Messina TM, and Brame CJ. Modeling interdisciplinarity in the biology classroom. In preparation.

Larkin L, Phillips J, Brame CJ, and Robinson LC. Serine 243 is an inhibitory phosphorylation site in the CK1 protein kinase Yck2. In preparation.

Brame CJ, Pruitt WM, and Robinson LC. A molecular genetics laboratory course applying bioinformatics and cell biology in the context of original research. CBE—Life Sciences Education 7: 410-421, 2008.

Davies SS, Amarnath V, Brame CJ, Boutaud O, and Roberts LJ, II. Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin g-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry. Nature Protocols 2: 2079-2091, 2007.

Webb DJ, Schroeder MJ, Brame CJ, Whitmore L, Shabanowitz J, Hunt DF,  and Horwitz, AR. Paxillin phosphorylation sites mapped by mass spectrometry. J. Cell Science 118: 4925-4929, 2005.

Moran MF, White F, Marto J, Brame CJ, Ornatsky O, Ross M, Toledo-Sherman LM, Castro A, Duewel H, Hosfield C, Orsi C, Topaloglou T, Figeys D, Caldwell-Busby J, and Stover DR. Phospho-proteomics in drug discovery and development. Chapter in: Protein Tyrosine Kinase Inhibitors in Cancer Therapy. D. Fabbro and F. McCormick, eds. Humana Press, 2004.

Marto JA, Brame CJ, Ficarro SB, White FM, Shabanowitz J, and Hunt DF. Sequence analysis: Low energy MS/MS-Peptide Interpretation. Chapter in: Encyclopedia of Mass Spectrometry, Volume 2: Biological Applications. R. Caprioli and M. Gross, eds. Elsevier, 2004.

Marto JA, Brame CJ, Ficarro SB, White FM, Shabanowitz J, and Hunt DF. Chemical derivitization for peptide sequence analysis and consideration for sequence analysis of peptides subjected to posttranslational modifications. Chapter in: Encyclopedia of Mass Spectrometry, Volume 2: Biological Applications. R. Caprioli and M. Gross, eds. Elsevier, 2004.

Brame CJ, Moran MF, and McBroom-Cerajewski, LD. A mass spectrometry-based method for distinguishing between symmetrically and asymmetrically dimethylated arginine residues. Rapid Communications in Mass Spectrom. 18: 877-81, 2004.

Brame CJ, Boutaud O, Davies S, Yang T, Oates JA, Salomon RG, Roden DM, Morrow JD, and Roberts LJ, II. Modification of proteins by isoketal-containing oxidized phospholipids. J. Biol. Chem. 279: 13447-51, 2004.

Zarling AL, Luckey CJ, Marto JA, White FM, Brame CJ, Evans AM, Lehner PJ, Cresswell P, Shabanowitz J, Hunt DF, and Engelhard VH. Tapasin is a facilitator, not an editor, of class I MHC peptide binding. J Immunol. 171: 5287-95, 2003.

Davies SS, Brame CJ, Boutaud O, and Roberts LJ,
II. Measurement of isoketal protein adducts by liquid chromatography
electrospray tandem mass spectrometry. Chapter in: Methods in Biological
Oxidative Stress
. K. Hensley and RA Floyd, eds., pp 127-136, 2003.

Harnpicharnchai P, Jakovljevid J, Horsey E, Miles T, Roman J, Rout M, Meagher D, Imai B, Guo Y, Brame CJ, Shabanowitz J, Hunt DF, and Woolford JL, Jr. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell 8: 505-515, 2001.

Boutaud O, Li J, Chaurand P, Brame CJ, Marnett LJ, Roberts LJ, II, and Oates JA. Oxygenation of arachidonic acid by cyclooxygenases generates reactive intermediates that form adducts with proteins. Adv Exp Med Biol. 500:133-137, 2001.

Strahl BD, Briggs SD, Brame CJ, Caldwell JA, Koh SS, Ma H, Cook RG, Shabanowitz J, Hunt DF, Stallcup MR, and Allis CD. Methylation of histone H4 at arginine 3 occurs in vivo and is mediated by the nuclear receptor coactivator PRMT1. Curr. Biol. 11: 996-1000, 2001.

Boutaud O, Brame CJ, Chaurand P, Li J, Rowlinson SW, Crews BC, Ji C Marnett LJ, Caprioli RM, Roberts LJ, II, and Oates JA. Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid. Biochemistry 40: 6948-6955, 2001.

Mosammaparast N, Jackson KR, Guo Y, Brame CJ, Shabanowitz J, Hunt DF, and Pemberton LF. Nuclear import of histone H2A and H2B is mediated by a network of karyopherins, including Kap114p, Kap121p, Kap123p, and Kap95p. J. Cell. Biol. 153: 251-262, 2001.

Roberts LJ, II, Chen Y, Boutaud O, Davies SS, Morrow JD, Oates JA, and Brame CJ. Reactive products of the isoprostane pathway: isoketals and cyclopentenone A2/J2-isoprostanes. Chapter in: Advances in Prostaglanding and Leukotriene Research: Basic Science and New Clinical Applications. B. Samuelsson, R. Paoletti, GC Folco, E. Granstrom, and S. Nicosia, eds. Kluwer Academic Publishers, 2001.

Hsu J-Y, Sun Z-W, Li X, Reuben M, Tatchell K, Biship DK, Grushcow JM, Brame CJ, Caldwell JA, Hunt DF, Lin R, Smith MM, and Allis CD. Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. Cell 102: 279-291, 2000.

Reich EE, Zackert WE, Brame CJ, Chen Y, Roberts LJ, II, Hachey DL, Montine TJ, and Morrow JD. Formation of novel D-ring and E-ring isoprostane-like compounds (D-4/E-4-neuroprostanes) in vivo from docosahexaenoic acid. Biochemistry 29: 2376-2383, 2000.

Roberts LJ, II, Brame CJ, Chen Y, and Morrow JD. Formation of novel reactive products via the isoprostane pathway. In: Proc. 5th Internat. Conf. On Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Other Related Diseases. KV Honn, S Nigam, L Marnett, and E Dennis, eds., Plenum Publishers, 2000.

Salomon RG, Sha W, Brame CJ, Kaur K, Subbanagounder G, O’Neil J, Hoff HF, and Roberts LJ, II. Protein adducts of iso[4]levuglandin E2 in oxidized low density lipoprotein. J. Biol. Chem. 274: 20271-20280, 1999.

Brame CJ, Boutaud O, Oates JA, and Roberts LJ, II. Characterization of lysyl adducts formed by the -ketoaldehyde prostanoids LGE2/D2. Biochemistry 38: 9389-9396, 1999.

Roberts LJ, II, Salomon RG, Morrow JD, and Brame CJ. New developments in the isoprostane pathway: identification of novel highly reactive -ketoaldehydes (isolevuglandins) and characterization of their protein adducts. FASEB J. 13: 1157-1168, 1999.

Roberts LJ, II, Brame CJ, Chen Y, Morrow JD, and Salomon RG. Formation of reactive products of the isoprostane pathway: isolevuglandins and cyclopenteneone isoprostanes. Adv. Exp. Med. & Biol. 469: 335-341, 1999.

Brame CJ, Salomon RG, Morrow JD, and Roberts LJ, II. Identification of extremely reactive -ketoaldehydes as products of the isoprostane pathway. J. Biol. Chem. 274: 13139-13146, 1999.

Roberts LJ, II, Brame CJ, Chen Y, and Morrow JD. Novel eicosanoids: Isoprostanes and related compounds. Methods Mol. Biol. 120: 257-285, 1999.

Morrow JD, Chen Y, Brame CJ, Yang J, Sanchez SC, Xu J, Zackert WE, Awad JA, and Roberts LJ, II. The isoprostanes: unique prostaglandin-like products of free radical-catalyzed lipid peroxidation. Drug Metab. Rev. 31, 117-139, 1999.

Subbanagounder G, Salomon RG, Murthi KK, Brame CJ, and Roberts LJ, II. Total synthesis of iso[4]-levuglandin E2. J. Org. Chem. 62: 7658-7666, 1997.

Mary E. Keithly, Cynthia J. Brame, Mark A. Woelfle, and Katherine L. Friedman. Blending it up: Active learning in a STEM classroom through the use of on-line materials. Presented at the American Chemical Society annual meeting, August 2015.

Gaurav Shah and Cynthia J. Brame. Isolation and phosphorylation analysis of Yck2, a CK1 protein kinase. Presented at the Centenary Student Research Forum, April 2012; awarded third place in the Natural Science oral competition.

Harry Chiang and Cynthia J. Brame. Does the RD pocket contribute to regulation of CK1 protein kinases? Presented at the Centenary Student Research Forum, April 2012.

MK Orsulak, Priscilla Hawthorne, and Cynthia J. Brame. Retinoid-containing anti-aging cosmiceuticals do not exhibit photocarcinogenicity in C57Bl/6 mice. Presented at the Centenary Student Research Forum, April 2012.

Wesley Carlisle and Cynthia J. Brame. Integration of mathematical approaches into the cell biology curriculum. Presented at the Centenary Student Research Forum, April 2011; awarded first place in the Humanities and Social Science oral competition.

Joshua Phillips, Lauren C. Larkin, Lucy C. Robinson, and Cynthia J. Brame. Ser243 in the activation loop of the Yck2 CK1 protein kinase may be an inhibitory phosphorylation site. Poster presented at 2011 Experimental Biology annual meeting, April 2011, and the Centenary Student Research Forum, April 2011

Lauren C. Larkin, Lucy C. Robinson, and Cynthia J. Brame. Investigation of a potential tyrosine phosphorylation site in the activation loop of a yeast casein kinase 1 protein kinase. Poster presented at 2011 Experimental Biology annual meeting, April 2011. Poster also presented at Student Research Forum, April 2011; awarded second place in poster presentations.

Matthew N. Blam, Troy C. Messina, Cynthia J. Brame, and Lucy C. Robinson. Molecular modeling of casein kinase 1. Poster presented at 241st National American Chemical Society meeting, March 2011.

S. Elise McMahen, Wesley Carlisle, Jonathan Carrere, Marci McMahen, Lucy C. Robinson, and Cynthia J. Brame. Does phosphorylation of a conserved tyrosine regulate CK1 activity? Poster presented at Student Research Forum, April 2010.

Ahmad Azzawe, Chris Bryan, Bethany Joseph, MK Orsulak, Taylor Pahls, Joshua Phillips, Lucy C. Robinson, and Cynthia J. Brame. Does phosphorylation of an activation loop serine inhibit CK1 activity? Poster presented at Student Research Forum, April 2010.

Dallas Krentzel, Noor Azzawe, Michaela Berg, Caitlin Cavarra, John Cefalu, Jordan Day, Rob Grant, Sarah Kuruvilla, Ruth Litwinowicz, Colin McRae, Marco Rajo, Kelly Reed, Tyler Smith, Garrett Vick, Heather Wensler, Lucy C. Robinson, and Cynthia J. Brame. Investigation of the conserved RD pocket in the CK1 protein kinase family. Poster presented at Student Research Forum, April 2010; awarded third place in poster presentations.

Jessica L. Miller, Lucy C. Robinson, and Cynthia J. Brame. Investigation of a conserved serine residue in a yeast casein kinase 1 protein kinase. Poster presented at 2009 American Society of Biochemistry and Molecular Biology Student Poster Competition, April 2009. Poster also presented at Student Research Forum, April 2009.

Jordan Day, Lucy C. Robinson, and Cynthia J. Brame. Investigation of a conserved tyrosine residue in a yeast casein kinase 1 protein kinase. Poster presented at 2009 American Society of Biochemistry and Molecular Biology Student Poster Competition, April 2009. Poster also presented at Student Research Forum, April 2009; awarded third place in poster presentations

Spencer Saulsbury, Cynthia J. Brame, and Lucy C. Robinson. Does Yck1/2 phosphorylation affect septin-dependent compartmentalization in yeast cells? Poster presented at 2009 American Society of Biochemistry and Molecular Biology Student Poster Competition, April 2009. Poster also presented at Student Research Forum, April 2009.

Jillian A. Kennedy, Lucy C. Robinson, and Cynthia J. Brame. Understanding the Differential Efficiency of Palmitoylation of Two C-terminal Cys Residues. Poster presented at the Student Research Forum, April 19, 2007. Awarded first place in poster presentations.

Jillian A. Kennedy, Elizabeth M. Wilson, Lucy C. Robinson, and Cynthia J. Brame. The Effect of Deletion of Four Consecutive Histidines on Yeast Casein Kinase 2 Function. Poster presented at Student Research Forum, April 21, 2006.

Talicia Johnson, Cheynita Metoyer, Lucy C. Robinson, and Cynthia J. Brame. Does instability of a mutant Yck2 protein require Yck2 kinase function? The effect of a kinase domain mutation in an unstable Yck2 mutant. Poster presented at Student Research Forum, April 21, 2006.

Kelly Waterhouse, Julia Reynolds, Lucy C. Robinson, and Cynthia J. Brame. Does deletion of a large glutamine/alanine-rich sequence alter function or localization of yeast casein kinase 2? Poster presented at Student Research Forum, April 21, 2006. Awarded second place in poster presentations.

Brittany Bjerke, Lucy C. Robinsin, and Cynthia J. Brame. The effects of the conversion of a lysine to an arginine in the catalytic site of Yck2 in Saccaromyces cerevisiae. Oral presentation at Student Research Forum, April 21, 2006.

Travis Reece, Michelle McNamara, Allyson Sandifer, Lucy C. Robinson, and Cynthia J. Brame. Membrane localization of Yeast Casein Kinase 2 by palmitoylation: Does distance from the carboxyl terminus matter? Oral presentation at Student Research Forum, April 21, 2006.

Brett Martin, Courtney Rome, Jennifer Wilson, Ashley Williams, Lucy C. Robinson, and Cynthia J. Brame. Effects of the deletion of 17 amino acids from the carboxyl terminus of Yeast Casein Kinase 2. Oral presentation at Student Research Forum, April 21, 2006. Awarded first place in oral presentations.

Beth Allen, Lucy C. Robinson, and Cynthia J. Brame. The effect of Yck phosphorylation of SEC4 on vesicle function with the plasma membrane. Poster presentation at Student Research Forum, April 2005.

  • Louisiana Board of Regents Support Fund Educational Enhancement Grant for “Learning to think like a scientist: Using differential centrifugation and phase contrast microscopy in a multi-week, inquiry-based project.” Awarded April 2009. $40,000 for one year.
  • Centenary Research Professorship in Natural Sciences, 2008-2010. Investigation of lipid peroxidation induction of protein misfolding in a cell culture model of Parkinson’s disease. $3000/year for two years.
  • Centenary Student/Faculty Summer Research Grant, 2008. Student investigations of Yck2 stability, function and localization using mutant alleles created by BIOL 313 students as part of the project described below.  Jordan Day (2010) and Jessica Miller (2010).
  • Centenary Research Professorship in Natural Sciences, 2006-2008. Student investigations of CK1 structure and function via mutagenesis followed by analysis of function and localization. $3000/year for two years.
  • Louisiana Board of Regents Support Fund Educational Enhancement Grant for “Enhancement of Biomolecular Tools at Centenary College” in collaboration with Dr. Scott Chirhart. Awarded April 2006. $47,802 for one year.
  • Louisiana Board of Regents Support Fund Educational Enhancement Grant for “Proteomics in the Undergraduate Curriculum.” Awarded April 2006. $47,803 for one year.
  • National Science Foundation Grant for “Identification of substrates for the yeast Yck2 protein kinase” in collaboration with Dr. Lucy Robinson. Awarded September 2005. $370,838 for three years.
  • Louisiana Board of Regents Grant within Research and Development Program and Research Competitiveness Subprogram for “Identification of substrates for the yeast Yck2 protein kinase” in collaboration with Dr. Lucy Robinson. Awarded June, 2005. $66,920 for two years.
  • Faculty Summer Research Grant, Summer 2005.
  • Patent granted: Methods of Detecting Protein Arginine Methytransferase, and Uses Related Thereto. United States Patent 6,747,273, granted June 8, 2004.
  • Mattie Allen Broyles Inaugural Year Research Chair, Sept. 2003-Sept. 2004.
  • Dissertation Enhancement Award. Grant awarded by Vanderbilt Graduate School for expansion of thesis work, 1998.
  • Predoctoral Fellowship, Pharmaceutical Research and Manufacturers of America (PhRMA) Foundation, 1997-1998.
  • Vanderbilt University Graduate School Fellowship, 1994-1998.
  • Interdisciplinary Graduate Program Fellowship, Vanderbilt University, 1994-1995.
  • Phi Beta Kappa, academic honor society, 1994.
  • Omicron Delta Kappa, honor society for leadership, 1994.
  • Barry Goldwater Scholarship for potential in science research, 1993-1994.

COURSES TAUGHT

Biochemistry

Principles and methods of biology

Cell biology with lab

Genetics with lab

Advanced cell biology

Biology of cancer

Techniques in molecular biology

 

PERSONAL INTERESTS

Growing, cooking, and eating food

 

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